Investigation Title	Protein Expression for TCGA Uterine Carcinosarcoma (UCS) samples using MDA Reverse Phase Protein Array (RPPA)							
Experimental Design	is_expressed_design							
Experimental Design Term Source REF	MGED Ontology	MGED Ontology						
Experimental Factor Type	disease_state_design	 						
Experimental Factor Type Term Source REF	MGED Ontology							
								
Person Last Name	Mills							
Person First Name	Gordon							
Person Mid Initials	B							
Person Email	gmills@mdanderson.org							
Person Phone	713-563-4200							
Person Address	"1400 Pressler Street, Unit Number: 1410, Houston, TX 77030"							
Person Affiliation	The University of Texas MD Anderson Cancer Center							
Person Roles	PI							
								
Date of Experiment	09/12/2014							
								
Public Release Date	11/05/2014							
								
Experiment Description	Protein Expression for TCGA UCS samples using Reverse Phase Protein Array by the Univ. of Texas MD Anderson Cancer Center RPPA Core Lab.						
								
Protocol Name	mdanderson.org:protein_extraction:MDA_RPPA_Core:01	mdanderson.org:protein_denature:MDA_RPPA_Core:01	mdanderson.org:serial_dilution:MDA_RPPA_Core:01	mdanderson.org:slide_print:MDA_RPPA_Core:01	mdanderson.org:antibody_probe:MDA_RPPA_Core:01	mdanderson.org:slide_scan:MDA_RPPA_Core:01	mdanderson.org:supercurve:MDA_RPPA_Core:01	mdanderson.org:protein_normalization:MDA_RPPA_Core:01
Protocol Type	protein_extraction	protein_denature	serial_dilution	slide_print	antibody_probe	slide_scan	supercurve	protein_normalization
Protocol Term Source REF	NCI EVS	NCI EVS	NCI EVS	NCI EVS	NCI EVS	NCI EVS	NCI EVS	NCI EVS
Protocol Description	protein extracted from shipped portions sent by BCR. 	denature protein using 1% SDS	two-fold serial dilution using dilution bufer	print (Aushon Biosystems 2470 Arrayer) dillutions on slide (Grace Bio-Labs Oncyte Avid nitrocellulose film slides)	wash antibody over slide (Dako stainer)	scan slide using a scanner (Cannon Scan 9000F)	ran SuperCureve R-package on data	Loading correction
Protocol Parameters								
								
SDRF Files	mdanderson.org_UCS.MDA_RPPA_Core.sdrf.txt							
Term Source Name	MGED Ontology	NCI EVS	NCBI Taxonomy					
Term Source File	http://mged.sourceforge.net/ontologies/MGEDontology.php	http://evs.nci.nih.gov/	http://www.ncbi.nlm.nih.gov/Taxonomy/taxonomyhome.html/					
Term Source Version	1.3.1.1	2010-09	2010-09					
